Solid Phase Peptide Synthesis Capping Procedure HEADING_TITLE

Standard Capping Procedure

  1. Filter and wash the resin several times with DMF.
  2. Suspend the resin in a DMF solution containing acetic anhydride (50 equivalent based on resin substitution) and pyridine (50 equivalents based on resin substitution).  DIPEA may be substituted for the pyridine.
  3. Gently shake at room temperature for 30 minutes.
  4. Filter and wash the resin with DMF.
  5. Perform a Kaiser test.  If the Kaiser test is not negative, repeat the capping procedure.