Monoclonal Antibody Production Packages HEADING_TITLE

AAPPTec offers the following packages for custom monoclonal antibody producting.  The times list are average times for delivery.  Actual times will vary depending on the difficulty of producing the monoclonal antibodies.

Package Options Service Items Delivery Time
Monoclonal Package 1 (From protein to ascites purification)
  • Protein checked by SDS-PAGE
  • Immunization and sera collection
  • ELISA identification
  • Fusion and hybridoma development
  • Isotype determination
  • Ascites production and purification (2 clones)
  • 50 μl Pre-immune sera (1:10 dilution)
  • 2-5 clones
  • 2-5 mg Purified antibodies (2 clones)
  • ELISA result
  • Isotype determination result
  • Project report
18-24 weeks
Monoclonal Package 2 (From peptide design to ascites purification)
  • Peptide design and synthesis (12-22aa, 85%)
  • Peptide conjugation
  • Immunization and sera collection
  • ELISA identification
  • Fusion and hybridoma development
  • Isotype determination
  • Ascites production and purification (2 clones)
  • 2-3 mg Peptides
  • 50μl Pre-immune sera  (1:10 dilution)
  • 2-5 Clones
  • 2-5mg Purified antibodies (2 clones)
  • ELISA result
  • Isotype determination result
  • Project report
20-26 weeks
Monoclonal Package 3(From plasmid to ascites purification)
  • Protein expression and purification
  • Immunization and sera collection
  • ELISA identification
  • Fusion and hybridoma development
  • Isotype determination
  • Ascites production and purification (2 clones)
  • 1 mg Protein
  • 50 μl Pre-immune sera (1:10 dilution)
  • 2-5 clones
  • 2-5 mg Purified antibodies (2 clones)
  • ELISA result
  • Isotype determination result
  • Project report
20-26 weeks
Monoclonal Package 4 (From cDNA to ascites purification)
  • Gene amplification
  • Vector construction
  • Protein expression and purification
  • Immunization and sera collection
  • ELISA identification
  • Fusion and hybridoma development
  • Isotype determination
  • Ascites production and purification (2 clones)
  • Recombinant vector
  • 1 mg Protein
  • 50 μl Pre-immune sera (1:10 dilution)
  • 2-5 clones
  • 2-5 mg Purified antibodies (2 clones)
  • ELISA result
  • Isotype determination result
  • Project report
22-26 weeks
Monoclonal Package 5 (Phospho-specific monoclonal antibodies)
  • Peptide design and synthesis (12-22aa, 85%)
  • Peptide conjugation
  • Immunization and sera collection
  • ELISA identification
  • Fusion and hybridoma development
  • Isotype determination
  • Ascites production and purification (2 clones)
  • 2-3 mg Peptides (unmodified and phospho)
  • 50 μl Pre-immune sera (1:10 dilution)
  • 2-5 Clones
  • 2-5 mg Purified antibodies (2 clones)
  • ELISA result
  • Isotype determination result
  • Project report
21-26 weeks
Monoclonal Package 6 (Western guaranteed monoclonal antibodies)
  • Peptide design and synthesis (12-22aa, 85%)
  • Peptide conjugation
  • Immunization and sera collection
  • ELISA and Western blot identification
  • Fusion and hybridoma development
  • Isotype determination
  • Ascites production and purification (2 clones)
  • 2-3 mg Peptides (unmodified and phospho)
  • 50 μl Pre-immune sera (1:10 dilution)
  • 2-5 Clones
  • 2-5 mg Purified antibodies (2 clones)
  • ELISA and Western blot results
  • Isotype determination result
  • Project report
20-26 weeks

Custom antibody