Custom Monoclonal Antibody Production HEADING_TITLE

Custom Monoclonal Antibodies

Featured Custom Monoclonal Antibody Production Services

Antigen Preparation

  • Peptide design
  • Peptide synthesis
  • Peptide conjugation
  • Protein expression

Custom Monoclonal Antibodies

  • Application guaranteed antibody (ELISA, Western blot, IHC)
  • Modification-specific antibody (phosphorylated, methylated, acetylated)

Antibody Purification

Antibody Identification

  • ELISA
  • Dot blot
  • Western blot
  • Immunohistochemistry (IHC)

Antibody Labeling

  • Horseradish peroxidase (HRP)
  • Biotin

AAPPTec offers a variety of monoclonal antibody production packages. These packages cover all antibody production steps including antigen preparation, immunization, sera collection and antibody identification.

Custom Monoclonal Antibody Production Services

Peptide Services Time
Peptide Design 1-2 days
Peptide Synthesis 2 weeks
Peptide Conjugation (KLB/BSA/OVA, 5 mg or less) 2-3 days
   
Protein Services Time
Gene Synthesis 3-4 weeks
Gene Amplification (Template provided by customer, 1000 bp or less) 2-3 weeks
Vector Construction 2-3 weeks
Recombinant Selection 1 week
Protein Expression and Purification 2-3 weeks
   
Monoclonal Antibody Services Time
Protein Checked by SDS-PAGE 2-3 days
Pre-immune Sera Collection 1 day
Immunization and Sera Collection (4 mice) 6-10 days
ELISA Identification 2-3 days
Fusion and Hybridoma Development 10-16 weeks
Isotype Determination 1 week
Ascites Production (3 mice) 3-4 weeks
Roller Bottle Cell Culture (1 L) 5-7 weeks
Prescreen (ELISA) 2-3 days
Prescreen (Western blot) 1 week
Prescreen (Dot blot) 1 week
Clone Maintenance (1 clone) 1 week
Clone Storage (1 clone) 1 week
   
Antibody Purification Services   Time
 Saturated Ammonium Sulfate Precipitation (60 mL or less) 3-5 days 
 Protein A/G Affinity Purification (60 mL or less)  3-5 days
 Antigen Affinity Column Preparation  2-3 days
 Antigen Affinity Purification 3-5 days 
   
 Antibody Identification Services  Time
 ELISA Identification (1-10 samples) 2-3 days 
 Cell Extraction (1 sample) 2-3 days 
 Tissue Extraction (1 sample) 2-3 days 
 Protein Concentration Assay (1 sample) 2-3 days 
 Western blot Identification (1-8 samples) 1 week 
 Dot blot Identification (1-10 samples) 2-3 days 
 IHC Identification (section provided by customer, 1 photo) 1 week 
   
 Antibody Labeling Services  Time
 HRP Labeling (5 mg or less) 1-2 weeks 
 Biotin Labeling (5 mg or less) 1-2 weeks 

 

Go To Monoclonal Antibody Production Packages

Custom antibody