Custom Polyclonal Antibody Production HEADING_TITLE

Custom Polyclonal Antibodies

Featured Polyclonal Antibody Production Services

Antigen Preparation (Polyclonal Antibody Production)

  • Peptide design
  • Peptide synthesis
  • Peptide conjugation
  • Protein expression
  • Custom Polyclonal Antibodies
  • Application guaranteed antibody (ELISA, Western blot, IHC)
  • Modification-specific antibody (phosphorylated, methylated, acetylated)


Antibody Purification (Polyclonal Antibodies)


Antibody Identification (Polyclonal Antibodies)

  • ELISA
  • Dot blot
  • Western blot
  • Immunohistochemistry (IHC)

Antibody Labeling (Polyclonal Antibodies)

  • Horseradish peroxidase (HRP)
  • Biotin

AAPPTec offers a variety of polyclonal antibody production packages. These packages cover all antibody production steps including antigen preparation, immunization, sera collection and antibody identification.

Custom Polyclonal Antibody Production Services

Peptide Services

Time

Peptide Design 1-2 days
Peptide Synthesis 2 weeks
Peptide Conjugation (KLB/BSA/OVA, 5 mg or less) 2-3 days
   

Protein Services

Time

Gene Synthesis 3-4 weeks
Gene Amplification (Template provided by customer, 1000 bp or less) 2-3 weeks
Vector Construction 2-3 weeks
Recombinant Selection 1 week
Protein Expression and Purification 2-3 weeks
   

Polyclonal Antibody Services

Time

Protein Checked by SDS-PAGE 2-3 days
Pre-immune Sera Collection 1 day
Immunization and Sera Collection (2 rabbits) 10 weeks
Immunization and Sera Collection (2 rabbits, fast) 6-8 weeks
ELISA Identification 2-3 days
Prescreen(ELISA) 2-3 days
Prescreen (Western Blot) 1 week
Prescreen (Dot Blot) 1 week
Additional Rabbit (1 rabbit) 6-10 weeks
Animal Maintenance (1 rabbit) 1 week
Additional Bleed (10 mL, 1 rabbit) 1 week
Extension Boost and Bleed (10 mL, 1 rabbit) 1 week
Immunization and Sera Collection (1 goat) 20 weeks
Immunization and Sera Collection (2 rats) 10 weeks
Immunization and Sera Collection (4 mice) 10 weeks
Immunization and Sera Collection (2 guinea pigs) 11 weeks

 

Go To Custom Polyclonal Antibody Production Packages

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