Technical Bulletins HEADING_TITLE

AAPPTec General Procedures, Protocols, and Technical Bulletins for Solid Phase Peptide Synthesis

These are recommended general procedures for preparing peptides by solid phase methods utilizing AAPPTec products.   

Peptide Synthesis Resins, Loading and Cleavage Procedures

1035  Aminomethyl Resin

1039  Carboxypolystyrene

1027  2-Chlorotritylchloride Resin

1029  Amino Acid-2-Chlorotrityl Resin

1175  DHP Resin

1042  Hydroxymethylpolystyrene

1046  Knorr-2-Chlorotrityl Resin

1047  MBHA Resin

1048  Merrifield Resin

1049  Oxime Resin

1051  PAM Resin

1054  Fmoc-Rink Amide AM Resin

1092  Sieber Amide Resin

1073  Wang Resin

1034  Fmoc-Amino Acid Wang Resins

1183  Preparing Low-Loading Fmoc-Amino Acid Wang Resins From High Substitution Wang Resin

1074  Weinreb Resin

1156  Use of Polystyrene Resins in Continuous Flow Synthesizers

1198  Resin Loading Measurement by Fmoc Cleavage

Amino Acid and Peptide Deprotection Procedures

General Protocols

1163  Removal of Boc Protecting Group

1140  Standard Fmoc Deprotection-Coupling Procedure

1173  Fmoc Removal with DBU

1162  Standard Removal of Z Protecting Group

1144  Removal of Allyl-based Protecting Groups; Aloc and Allyl Esters

1207  Removal of t-Butyl-based Protecting Groups in Solution

1178  Glycopeptide Synthesis and Deprotection


1026  Fmoc-Arg(Pmc)-OH vs Fmoc-Arg(Pbf)-OH

1087  Cleaving Mtr Group from Arginine


1161  Removal of N-Xan Groups


1123  Removal of S-Acetamidomethyl Groups

1180  Cys(Acm) Deprotection and On-Resin Peptide Cyclization

1124  Removal of S-Benzyl, S-p-Methylbenzyl, and S-p-Methoxybenzyl Groups

1164  Deprotection of Cys(4-MeBzl)

1125  Removal of S-t-Butyl Groups

1127  Removal of S-Trityl Groups

1128  Removal of S-Fluorenylmethyl Groups


1150  Removal of Dnp Protecting Groups From Histidine


1169  Removal of 2-Cl-Z

1182  Removal of Dde and ivDde Protecting Groups

1181  Selective Removal of Mtt Protecting Group From Amines


1153  Post Cleavage Reduction of Met(O)


1154  Deprotection of Thr(TBDMS) and allo-Thr(TBDMS)


1151  Deformylation of Trp(CHO)


1143  Removal of 2-Br-Z Groups from Tyrosine

Peptide Coupling Protocols

1147  Coupling Dialkylglycines

1177  Coupling to N-Methyl Amino Acids

1179  Fmoc-AA-OPfp Ester Coupling

1095  BOP  (Castro’s Reagent)

1096  Benzotriazole-1-yloxy-tris-pyrrolidinophosphonium Hexafluorophosphate (PyBOP)

1102  Bromo-tris-pyrrolidino-phosphonium hexafluorophosphate (PyBrOP)

1170  DEPBT [3-(Diethylphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one]

1104  Dicyclohexylcarbodiimide (DCC)

1105  Diisopropylcarbodiimide (DIC)

1098  HOBt (N-Hydroxybenzotriazole)

1099  EDC (Water-Soluble Carbodiimide)

1205  HATU

1100  HBTU

1206  HCTU

1171  TDBTU (O-3,4-Dihydro-4-oxo-1,2,3-benzotriazine-3yl-1,1,3,3-tetramethyluronium tetrafluoroborate)

1101  TBTU

1103  N,N,N’,N’-Tetramethyl(succinimido)uronium Tetrafluoroborate (TSTU)

1196  Activation and Coupling of Biotin

1202  Phosphoamino Acids

1208  Methylated Lysines

Solid Phase and Solution Phase Test Protocols

1188  Kaiser Test (Ninhydrin Test)

1189  Tests for Unprotected Proline

1191  General Tests for Free Amines

1186  Determining Chiral Purity of Amino Acids

1190  Ellman’s Test

Solid Phase Peptide Synthesis Cleavage Cocktails

1192 “Odorless” TIS Cleavage Cocktail (Reagent B)

1157  Cleavage Cocktail for Methionine Containing Peptides (Reagent H)

1193  Cleavage Cocktail for Peptides Containing Cys, Met, Trp and Tyr Residues (Reagent K)

1167  Low Odor Cleavage Cocktail (Reagent L)

1168  Cleavage Cocktail for Peptide Containing Arg Residues (Reagent R)

Peptide Purification and Handling

1172  HPLC Purification of Peptides

1194  Storage and Handling of Peptides

1195  Dissolving Purified Peptides

1085  Removing Trifluoroacetic Acid (TFA) From Peptides

Additional Technical Information

1114  Fmoc-Rink Amide Linker

1121  PNA Synthesis

1137  Converting CHA and DCHA Salts to Free Acids

1176  Ring Closing Metathesis and Hydrogenation on Resin

1184  Attaching Boc Protecting Group With Boc-ON

1185  Reduction of Disulfide Bonds

1197  Click Chemistry on Resin

1203  On-Resin Peptide Labeling with FITC

1204  On-Resin Peptide Labeling with FAM

1209  Allyl Ester Deprotection and On-Resin Cyclization