MAP Resins HEADING_TITLE

Multiple Antigen Peptide (MAP) Resins

Multiple Antigen Peptide (MAP) resins provide a branching core matrix for preparing synthetic peptide antigens. MAP peptides produced on these resins are utilized as synthetic vaccines and produce anti-peptide antibodies.  Incorporation of a cysteine residue into the core matrix allows MAPs with different epitopes to be linked by a disulfide bond.

MAP resins are available in bulk quatities from AAPPTec.  Please send an e-mail to sales@aapptec.com for a quotation or use the On-Line Quote Request.

Multiple Antigen Peptide System

Multiple Antigen Peptides (MAPs) were developed by J.P. Tam to produce high-titer anti-peptide antibodies and synthetic peptide vaccines.  The MAP system utilizes radially branched lysine residues to form a backbone to which multiple peptide chains are attached.  Typically, the antigen sequences are synthesized on a lysine core attached to a solid phase synthesis resin.  Depending on the lysine core, the MAP will contain four (4-branch) or eight (8-branch) copies of the peptide epitope.  Generally, the lysine core accounts for less than 10% of the total molecular weight of the MAP.  The dense packing of multiple copies of the epitope and its high molar ratio produce a strong immunological response.  Therefore the multiple antigen peptide system does not need a carrier protein for conjugation.

Multiple Antigen Peptide References

J.P. Tam Proc. Nat. Acad. Sci. USA 1988, 85, 5409-13.

D. Posnett, H. McGrath, J.P. Tam J. Biol. Chem. 1988, 263, 1719-25.

J. P. Tam, Y.-A. Lu, Proc. Nat. Acad. Sci. USA 1989, 86, 9084-9088.

S. Araga, L. Xu, K. Nakashima, M. Villain, J.E. Blalock. FASEB Journal 2000, 14, 185-96.

C.Y. Wang, D.J. Looney, M.L. Li, A.M. Walfeld, J. Ye, B. Hosien, J.P. Tam, F. Wong-Staal Science 1991, 254, 285-8.

M Christodoulides, J. E. Heckels Microbiology 1994, 140, 2951-2960.

Brooks NA, Pouniotis DS, Sheng KC, Apostolopoulos V, Pietersz GA. Biochim Biophys Acta. 2010, 1798, 2286-2295.

 

 

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