Dissolving Purified Peptides HEADING_TITLE
  1. Dissolve basic peptides in a small amount of 30% acetic acid, then dilute to the required concentration.
  2. Dissolve acidic peptides in a small amount of 10% ammonium bicarbonate, then dilute to the required concentration.
  3. For hydrophobic peptides that do not dissolve in water, dissolve the peptide in the minimum amount of dimethylsulfoxide (DMSO) then dilute with water to the required concentration.
  4. Peptides that aggregate can be dissolved by adding 6 M urea, 6 M urea with 20% acetic acid or 6 M guanidine hydrochloride salt, then diluting to the required concentration.