Apex 396 Description
The Apex range provides an easy to use automated liquid handling system capable of both liquid and solid phase peptide synthesis and organic synthesis reactions. All reagents and reaction vessels are contained within the cabinet on the indexed aluminum alloy tabletop. There is no additional fume hood required.
Up to 96 compounds can be synthesized simultaneously within the Teflon™ reaction blocks, which are also available in 40-well, 16-well and 8-well formats for easy synthesis scale-up. In a 96-well reaction format, the average synthesis time for a 30 mer peptide is 20 minutes per peptide. Depending on the synthesis chemistry, the cycle time for amino acid addition can range from only 45 minutes to 1.5 hours.
The coaxial spray probe delivers solvent accurately down to 0.5 ml. The coaxial probe has two channels: one for solvent and the other for inert gas (either nitrogen or argon). During solvent aspiration, the removed solvent is replaced with inert gas; during solvent delivery, gas is maintained throughout synthesis. The probe is also responsible for liquid detection, which is identified by measuring the solvent dielectric constant. This ensures the probe enters the solvent just below the liquid level, minimizing probe exposure and therefore minimizing cross-contamination between precious samples.
Reaction vessels are emptied by positive nitrogen pressure, maintaining an inert environment and ensuring effective removal of all solvents and reagents from the bottom of the vessel; such emptying also keeps the sample resin at the bottom of each reaction vessel.
The Apex range is available in four models:
Apex 396-SC: with single arm and classic reactor
Apex 396-DC: with dual arm system and classic reactor
Apex 396-DC-FW-S: with dual arm system, classic reactor, fast-wash capability and single needle probe on second arm
Apex 396-DC-FW-M: with dual arm system, classic reactor, fast-wash capability and multi-needle probe on second arm
The Apex 396 is the biggest selling instrument of the aapptec range, which would be expected based on its reliability, robustness, ease of use and high performance. High quality pure peptides are synthesized easily and rapidly and all types of peptide and organic chemistry reactions can be performed in this highly flexible tabletop unit.
Key Features
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Multiple peptide synthesizers performing both liquid and solid phase chemistry
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1-96 compounds can be synthesized simultaneously
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Numerous amino acid and reaction vessel configurations
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Tabletop synthesizer
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Fully enclosed hood to contain fumes and unwanted odors
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Indexed tabletop allows maximum flexibility for reagent locations
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Automatic cleavage for Fmoc- chemistry
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Dilutor/syringe dispensing system with microliter accuracy
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Dual arm robotics available with either single- or multi-probe on second arm
Key Specifications
- Variable scale synthesis
- Variable speed vortex mixing of the reaction block, 300-1200 rpm
- Teflon™ reactors for non-stick surface and inert reaction environment
- Teflon™ reactor frits for sample filtration
- Septa-capped reaction wells to maintain pressure, prevent cross-contamination and minimize solvent loss
- Indexed aluminum allows the tabletop for flexible reagent locations
- Up to 6 bulk external solvent lines
- 350 ml and 800 ml bottles for reagents
- Two dilutors for solvent reagents on first arm (in all Apex models)
- Gear pump for solvent delivery from dilutors
- Accurate solvent delivery down to 0.5 l
- Coaxial spray probe used for solvent delivery and liquid detection
- Probe wash station to prevent cross-contamination
- Nitrogen-assisted positive pressure vessel emptying in less than 1 minute
- Nitrogen and solvent detection systems to prevent operation in absence of reagents
- Fast-wash capabilities in the dual arm Apex models, 1-1.5 minutes in 96-well reactor
- Intuitive software for easy use and programming – simple, point and click commands
Key Options
Optional accessories:
- Teflon™ reaction blocks: 96-well, 40-well, 16-well and 8-well available
- Resin dispenser: 35 mg or 70 mg capability available
- Monomer racks: 4x9 racks with 35 ml bottles, 8x12 racks with 10 ml bottles, 2x5 racks with 125 ml bottles and 5x6 racks with 125 ml bottles available. Custom racks are available.
- Microtiter blocks for delivery of sample to a 96-well microtiter plate
- Cleavage blocks for deposition of crude sample after cleavage from resin; available in 96 x 4 ml vials, 40 x 6 ml vials, 16 x 15 ml vials and 8 x 35 ml vials
Software Drivers
aapptec Win9000 software
Size
W: 36” (92 cm)
H: 36” (92 cm)
D: 30” (77 cm)
Weight: approximately 300-350 lbs (137-160 kg)
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